Plugin to Subtract Images?

[SUBSCRIBE IMAGEJ Tanja M]

Hello,

I am working a project where I have to compare/contrast the colour intensity between otherwise identical pixels from two images. Is there a plugin that will allow me to do this? Someone had mentioned a "subtract" plugin, but I have not been able to find this.

Any help would be great!

Tanja

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[Mark Hiner]

Hi Tanja,

Post by SUBSCRIBE IMAGEJ Tanja M
Someone had mentioned a "subtract" plugin, but I have not been able to

find this.

Press "L" to open the Command Finder <http://imagej.net/Command_Finder>.
You can then interactively search for commands.

You actually do not want the "Subtract" command here, as that subtracts a
fixed value from an image. Instead, use Image Calculator
<http://imagej.net/docs/guide/146-29.html#toc-Subsection-29.13> and select
the Subtract operation.

Hope that helps,
Mark

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[SUBSCRIBE IMAGEJ Tanja M]

Thank you Mark,

That did work.
Now is it possible to have ImageJ do this for numerous images (a few hundred) automatically?

Kind regards,

Tanja

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[Mark Hiner]

Hi Tanja,

Now is it possible to have ImageJ do this for numerous images (a few

Post by SUBSCRIBE IMAGEJ Tanja M
hundred) automatically?

Indeed it is! I actually just recently made a Wiki page with instructions
on doing so: http://imagej.net/Batch_Processing

Please let me know if you run into problems using the techniques on that
page.

Regards,
Mark

Post by SUBSCRIBE IMAGEJ Tanja M
Thank you Mark,
That did work.
Now is it possible to have ImageJ do this for numerous images (a few
hundred) automatically?
Kind regards,
Tanja
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