Merge channel change in size?

[venierr]

Hello,

I've taken some pictures of fluorescently labelled cells and am now trying
to merge the channels, create a composite and then a montage. I've done this
before for other pictures and have had no problem but this time, merging
channels changes the size of the picture and so the composite won't stack
with the individual channels. These pictures are TIFs and in the past I've
worked with JPGs - is that the problem? I don't want to sacrifice the
pictures by changing the format and there has to be a way for me to do all
this with the TIFs. Right?

Any help is appreciated.

Rose

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[Peter Haub]

Hi Rose

TIFs and JPGs make no difference. Once loaded the image is represented
inside IJ independent from the files storing format.

Are you using the < Merge Channels.. > function?
I checked the code of the function < Image / Color / Merge Channels.. >
and can not see any problem.
Resizing should not occur. The functions starts only if the width and
height of all images/channels are identical.

What is your exact procedure?

Peter

Post by venierr
Hello,
I've taken some pictures of fluorescently labelled cells and am now trying
to merge the channels, create a composite and then a montage. I've done this
before for other pictures and have had no problem but this time, merging
channels changes the size of the picture and so the composite won't stack
with the individual channels. These pictures are TIFs and in the past I've
worked with JPGs - is that the problem? I don't want to sacrifice the
pictures by changing the format and there has to be a way for me to do all
this with the TIFs. Right?
Any help is appreciated.
Rose
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[venierr]

Hi Peter,

I am using the merge channels function: Image>Color>Merge channels. It seems
that it's stacking the pictures instead of merging them into one so the
individual pictures are 1MB and then the merge is 2MB and says 1/2 and 2/2
although both pictures look the same. This is then preventing me from
stacking all three pictures.

Rose

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[Michael Schmid]

Hi Rose,

you have to deselect 'Create Composite' if you want to have a single
RGB image.
Note that the size of an RGB image is not the same as that of the
original image, tough: E.g., if you merge 8-bit images, 1 MB each,
the RGB will have 4 MB.

(by the way, not everyone on the list is named Peter ;-)

Michael
________________________________________________________________

Post by venierr
Hi Peter,
I am using the merge channels function: Image>Color>Merge channels. It seems
that it's stacking the pictures instead of merging them into one so the
individual pictures are 1MB and then the merge is 2MB and says 1/2 and 2/2
although both pictures look the same. This is then preventing me from
stacking all three pictures.
Rose

[venierr]

It worked! I can't believe it was that simple. That's a little embarrassing.
How come I've never had that problem before when I left it checked? What is
it doing exactly?

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[Jan Eglinger]

Hi Rose,

Post by venierr
It worked! I can't believe it was that simple. That's a little embarrassing.
How come I've never had that problem before when I left it checked? What is
it doing exactly?

To get more info on composite images, read:
http://rsbweb.nih.gov/ij/docs/menus/image.html#make-composite

The advantages of using color composites instead of RGB images are
explained in the ImageJ user guide:
http://rsbweb.nih.gov/ij/docs/guide/userguide-9.html#toc-Subsection--11

Hope that helps,
Jan