Measure distance between edges coming close to each other

[shauryasachdev]

I am trying to measure the distance between edges that are coming close to
each other in a sequence of images. Is this possible to do via ImageJ?

I have around 1000 images and I want to measure the distance between the
edges in each of them. The edges come close to each other in this sequence
of these 1000 images.

For reference I am attaching 4 images from the sequence of 1000 images I
have.

<Loading Image...>
<Loading Image...>
<Loading Image...>
<Loading Image...>

Is it possible to do this in one go and store the required data in a file or
possibly automate the process using a macro? Opening each image and manually
measuring the distance is not practical for my project. I am new to imageJ
and have only a little clue on how to proceed.

Could anyone please help me out here?

Thanking you in advance!



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[Jerome Mutterer]

Hi shauryasachdev
You could import all of your images as an image stack, draw a straight line
across a channel and that intercepts the object, then reslice the stack
accordingly. You will get a (line length)x1000 image on which measuring the
width of your object will be easier.

Relevant elements to achieve this are :

File>Import>Image Sequence... command
The Straight Line Tool (5th tool on the toolbar)
Image>Satck>Reslice [/]... command

Jerome.


I am trying to measure the distance between edges that are coming close to

each other in a sequence of images. Is this possible to do via ImageJ?

I have around 1000 images and I want to measure the distance between the
edges in each of them. The edges come close to each other in this sequence
of these 1000 images.

For reference I am attaching 4 images from the sequence of 1000 images I
have.

<http://imagej.1557.x6.nabble.com/file/n5009632/test001557.jpg>
<http://imagej.1557.x6.nabble.com/file/n5009632/test005263.jpg>
<http://imagej.1557.x6.nabble.com/file/n5009632/test009413.jpg>
<http://imagej.1557.x6.nabble.com/file/n5009632/test010445.jpg>

Is it possible to do this in one go and store the required data in a file or
possibly automate the process using a macro? Opening each image and manually
measuring the distance is not practical for my project. I am new to imageJ
and have only a little clue on how to proceed.

Could anyone please help me out here?

Thanking you in advance!



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[shauryasachdev]

Thanks Jerome,

The method you mentioned is way more elegant than what I was trying before.
I implemented this method and now I able to measure the distance easily with
very little or zero error.

Shaurya



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