Sarah Mohamed
2014-09-16 10:15:10 UTC
Hi,
Can I manipulate with the image brightness before analysis using threshold?
I am trying to determine brown area stained with DAB using image J, i
tried to use threshold and color deconvolution. my images a dark can i
use photoshop to adjust background or adjust brightness (using levels or curves) ? or that will change the results?
Regards,Sarah M. Mosaad, M.Sc.
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html
Can I manipulate with the image brightness before analysis using threshold?
I am trying to determine brown area stained with DAB using image J, i
tried to use threshold and color deconvolution. my images a dark can i
use photoshop to adjust background or adjust brightness (using levels or curves) ? or that will change the results?
Regards,Sarah M. Mosaad, M.Sc.
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html