Pixel shift/image registration

Breukers, C (TNW)

2006-10-02 07:22:33 UTC

Dear David,

We had the same problem a while ago with our fluorescent images.

The cells in two channels were shifted a few pixels in x and y direction
due to the optical alignment of the system.

We used the color functions plugin: align RGB planes.

By using this tool it is rather easy to shift manually one channel pixel
by pixel with respect to the other.

Though if you have a lot of images this is not an ideal solution.

Gabriel Landini made this plugin, for download:
http://imagejdocu.tudor.lu/imagej-documenation-wiki/plugins/align_rgb_pl
anes

Personally I would like to know if there is also some kind of plugin or
algorithm to do it automatically.

Good luck,
Christian Breukers

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-----Original Message-----
From: ImageJ Interest Group [mailto:***@LIST.NIH.GOV] On Behalf Of
David Knecht-charter
Sent: maandag 2 oktober 2006 3:45
To: ***@LIST.NIH.GOV
Subject: Pixel shift/image registration

I have what appears to be a pixel shift between red and green
fluorescence images. I am trying to track down the source, but in
the meantime, is there an easy way in ImageJ to manually shift one
image a few pixels relative to the other to register them before/
after merging? It would be nice to be able to do this on the RGB
merged image, but I can't find any way to move one plane relative the
other as you can in Photoshop. Thanks- Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)